Efficient propagation of progressive multifocal leukoencephalopathy‐type JC virus in COS‐7‐derived cell lines stably expressing Tat protein of human immunodeficiency virus type 1
Identifieur interne : 000994 ( Main/Exploration ); précédent : 000993; suivant : 000995Efficient propagation of progressive multifocal leukoencephalopathy‐type JC virus in COS‐7‐derived cell lines stably expressing Tat protein of human immunodeficiency virus type 1
Auteurs : Souichi Nukuzuma ; Kazuo Nakamichi ; Masanori Kameoka ; Shigeki Sugiura ; Chiyoko Nukuzuma ; Isao Miyoshi ; Tsutomu Takegami [Japon]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2010-12.
English descriptors
- KwdEn :
- Aids patients, Archetype, Assay, Blackwell publishing asia, Cell clones, Cell growth, Cell lines, Cell lines stably, Cell proliferation, Cellular protein, Clin microbiol, Clone, Culture medium, Culture plates, Current study, Detectable level, Enzyme activity, Epithelial cells, Expression plasmid, Genomic, Glial cells, High incidence, Human polyomavirus, Human virus type, Incubation period, Khalili, Kobe institute, Microbiol immunol, Nukuzuma, Other cell clones, Parental, Parental cells, Parental cos7 cells, Previous study, Progressive multifocal, Progressive multifocal leukoencephalopathy, Proliferation characteristics, Propagation characteristics, Replication, Stable expression, Standard deviations, Time points, Transfected cells, Transfection, Untransfected cells, Useful model system, Viral, Viral genome, Viral genomic, Viral particles, Virol, Virus origin, Virus propagation.
- Teeft :
- Aids patients, Archetype, Assay, Blackwell publishing asia, Cell clones, Cell growth, Cell lines, Cell lines stably, Cell proliferation, Cellular protein, Clin microbiol, Clone, Culture medium, Culture plates, Current study, Detectable level, Enzyme activity, Epithelial cells, Expression plasmid, Genomic, Glial cells, High incidence, Human polyomavirus, Human virus type, Incubation period, Khalili, Kobe institute, Microbiol immunol, Nukuzuma, Other cell clones, Parental, Parental cells, Parental cos7 cells, Previous study, Progressive multifocal, Progressive multifocal leukoencephalopathy, Proliferation characteristics, Propagation characteristics, Replication, Stable expression, Standard deviations, Time points, Transfected cells, Transfection, Untransfected cells, Useful model system, Viral, Viral genome, Viral genomic, Viral particles, Virol, Virus origin, Virus propagation.
Abstract
The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV‐1 infection is strictly related to the activation of JC virus (JCV) propagation. In this report, propagation of PML‐type JCV in COS‐7‐derived cell lines stably expressing HIV‐1 Tat (COS‐tat cells) has been examined. In COS‐tat cells, production of viral particles and replication of genomic DNA were markedly increased compared to COS‐7 cells, as judged by HA and real‐time PCR analyses. These results demonstrate that COS‐tat cells provide a useful model system for studying HIV‐1 Tat‐mediated propagation of PML‐type JCV.
Url:
DOI: 10.1111/j.1348-0421.2010.00278.x
Affiliations:
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Le document en format XML
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<term>Cell growth</term>
<term>Cell lines</term>
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<term>Detectable level</term>
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<term>Glial cells</term>
<term>High incidence</term>
<term>Human polyomavirus</term>
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<term>Incubation period</term>
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<term>Microbiol immunol</term>
<term>Nukuzuma</term>
<term>Other cell clones</term>
<term>Parental</term>
<term>Parental cells</term>
<term>Parental cos7 cells</term>
<term>Previous study</term>
<term>Progressive multifocal</term>
<term>Progressive multifocal leukoencephalopathy</term>
<term>Proliferation characteristics</term>
<term>Propagation characteristics</term>
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<term>Stable expression</term>
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<term>Cell lines</term>
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<term>Cellular protein</term>
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<term>High incidence</term>
<term>Human polyomavirus</term>
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<front><div type="abstract" xml:lang="en">The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV‐1 infection is strictly related to the activation of JC virus (JCV) propagation. In this report, propagation of PML‐type JCV in COS‐7‐derived cell lines stably expressing HIV‐1 Tat (COS‐tat cells) has been examined. In COS‐tat cells, production of viral particles and replication of genomic DNA were markedly increased compared to COS‐7 cells, as judged by HA and real‐time PCR analyses. These results demonstrate that COS‐tat cells provide a useful model system for studying HIV‐1 Tat‐mediated propagation of PML‐type JCV.</div>
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<name sortKey="Miyoshi, Isao" sort="Miyoshi, Isao" uniqKey="Miyoshi I" first="Isao" last="Miyoshi">Isao Miyoshi</name>
<name sortKey="Nakamichi, Kazuo" sort="Nakamichi, Kazuo" uniqKey="Nakamichi K" first="Kazuo" last="Nakamichi">Kazuo Nakamichi</name>
<name sortKey="Nukuzuma, Chiyoko" sort="Nukuzuma, Chiyoko" uniqKey="Nukuzuma C" first="Chiyoko" last="Nukuzuma">Chiyoko Nukuzuma</name>
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